MCQ Practice Test - Chapter 9-BIOTECHNOLOGY : PRINCIPLES AND PROCESSES

Welcome to your Ncert - Class 12-Biology -Biotechnology : Principles and Processes

Instruction

Total Questions : 20

Total Marks : 20 x 4 = 80 Marks

Each Correct Question carries : 4 Marks

Each incorrect Question Carries : -1 Marks

Each Unattempted Question carry : 0 Marks

Be careful On Negative Markings

Polymerase chain reaction involves use of a DNA polymerase enzyme which is a

Terminal transferase

Thermolabile

Thermostable

Modular enzyme

None

Which of the following techniques is used to make numerous copies of a specific segment of DNA quickly and accurately?

Ligase chain reaction

Polymerase chain reaction

Translation

Transcription

None

Plants are more readily manipulated by genetic engineering than are animals because

a somatic plant cell can grow into a complete plant

plant genes do not contain introns

recombinant genes can be inserted into plant cells by microinjection

more vectors are available for transferring recombinant DNA into plant cells

None

Polymerase chain reaction (PCR) is used for

In vitro replication of specific DNA sequence using thermostable DNA polymerase

Separation of DNA fragments according to their size

In vitro synthesis of mRNAIn vivo synthesis of mRNA

In vivo replication of specific DNA sequence using thermostable DNA polymerase

None

DNA polymerase required for synthesizing copies of DNA at high temperature is obtained from

Bacillus megaterium

Thermoproteus species

Cephalosporium minimum

Thermus aquaticus

None

The goal of the polymerase chain reaction is to

Create many copies of a DNA molecules which is initially very rare.

Create many copies of messenger RNA molecules.

Speed up protein synthesis for the production new drugs.

Investigate the properties of organisms which normally grow at very high temperatures

None

Read the following statements carefully and select the correct option:

(A) Source of the restriction enzyme Hind III is E.coli

(B) In biolistic method of gene transfer, mircoparticles made up of gold or tungsten are coated with foreign DNA

(D) Primers are chemically synthesized oligonucleotides that are complementary to the regions of DNA in PCR.

How many of the above statement are correct?

None

Some foreign DNA fragment is attached to Cla I site of pBR 322. This recombinant vector is used to transition Escherichia coli. The cells subjected to transformation are plated on two different media, one containing ampicillin and the other containing tetracycline. The transformed cells containing the recombinant vector will

Grow on ampicillin but not on tetracycline containing medium

Grow on both, tetracycline and ampicillin containing media

Grow on tetracycline but not on ampicillin containing medium

Not grow on either tetracycline containing or ampicillin containing media

None

In order to induce the bacterial uptake of plasmids, the bacteria are made competent by treating them with

Magnesium chloride

Sodium chloride

Potassium chloride

Calcium chloride

None

10.

Which of the following steps should be performed by a person in order to visualise the bands of DNA fragments obtained from gel electrophoresis?

Person can see the bands without staining4

Staining with ethidium bromide followed by exposure to UV radiations

Staining with bromophenol blue followed by exposure to UV radiations

Exposure of DNA fragments to UV radiations

None

11.

Study the following statements regarding recombinant DNA technology and select the incorrect ones

(i) Taq polymerase extends the primers using the nucleotides provided in the reaction

(ii) Antibiotic resistance genes are considered as desirable genes in recombinant DNA technology

(iii) DNA fragments are separated according to their charge only, in agarose gel electrophoresis

(iv) Transformation is a procedure through which a piece of DNA is integrated into the genome of a host bacterium

(v) To produce higher yields of the desired protein, host cells can be multiplied in a continuous culture

(vi) Downstream processing is one of the steps of polymerase chain reaction

(i), (iii) and (vi)

(i), (iv) and (v)

(ii), (iii) and (v)

(i), (iii) and (v)

None

12.

The new strain of bacteria produced by biotechnology in bioremediation is

Pseudomonas putida

Saccharomyces cerevisiae

Bacillus subtilis

Escherichia coli

None

13.

Which of the following steps is/are catalysed by Taq polymerase in a PCR?

Annealing of primers to template DNA

Extension of primer end on template DNA

Denaturation of template DNA

All of these

None

14.

The process of making temporary/transitory opening in the cell membrane to introduce foreign DNA is ____

Chemical mediated genetic transformation

Particle gun

Microinjection

Electroporation

None

15.

Which of the following statements does not hold true for restriction enzyme ?

It is isolated from bacteriophages

It produces the same kind of sticky ends in different DNA molecules

It recognizes a palindromic nucleotide sequence

It is an endonuclease

None

16.

Restriction endonucleases are isolated from some bacteria. Their role in bacteria is

Defence against virus

Synthesis of proteins

Act as genetic material

Help in reproduction

None

17.

PCR proceeds in three distinct steps governed by temperature. The order of the steps is

Annealing, Synthesis, Denaturation

Denaturation, Synthesis, Annealing

Denaturation, Annealing, Synthesis

Synthesis, Annealing, Denaturation

None

18.

In a polymerase chain reaction after the denaturation step why the mixture needs to cool down to a lower temperature?

To permit specific annealing of the primers

To give a halt to the reaction mixture

To increase the activity of enzyme Taq polymerase

To obtain the multiple copies of the DNA

None

19.

After insertion of DNA segment within the sequence of Z-gene of bacteria, they are grown on chromogenic substrate. After the growth of bacterial colonies, they are identified as

i) Recombinants if colonies are blue- coloured.

ii) Recombinants if colonies are white coloured.

iii) Non-recombinants if colonies are blue- coloured.

iv) Non-recombinants if colonies are white coloured.

ii, iii

i, iv

i, ii

iii, iv

None

20.

In biotechnology application, a selectable marker is incorporated in a plasmid

To increase the expression of the gene of interest

To eliminate the non transformants

To increase the transformation efficiency

To increase its copy number

None

Curriculum

Ncert - class 12 - Biology - unit IX - BIOTECHNOLOGY- Notes, online test, practice test

Chapter 9-BIOTECHNOLOGY : PRINCIPLES AND PROCESSES

CHAPTER 10 BIOTECHNOLOGY AND ITS APPLICATIONS

MCQ Practice Test – Chapter 9-BIOTECHNOLOGY : PRINCIPLES AND PROCESSES

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