Ncert-Class12- Biology- Biotechnology and its Applications

Welcome to your Ncert-Class12- Biology- Biotechnology and its Applications

Instruction

Total Questions : 20

Total Marks : 20 x 4 = 80 Marks

Each Correct Question carries : 4 Marks

Each incorrect Question Carries : -1 Marks

Each Unattempted Question carry : 0 Marks

Be careful On Negative Markings

Select the correct option for the given statements X, Y, and Z.

X - A transgenic cow, Rosie produced human protein-enriched milk, which was a nutritionally more balanced product for human babies than natural cow milk.

Y- Milk produced by transgenic cow Rosie contains 2.4 gm protein/L.

Z- In the above-mentioned milk in Y statement, alpha-lactalbumin is present.

Statements X and Y are wrong and statement Z is correct

Statements X, Y, Z are true and statement Z gives correct explanation for X

Statements X and Y are correct and statement Z is wrong

Statements X, Y, Z are true and statement Z does not give correct explanation of X

None

All of the followings are associated with replacement principle of animal biotechnology except _______.

Improving experimental technique

Epidemiological studies

Computer models

Cell culture

None

Human insulin is being commercially produced from a transgenic species of _______________.

Saccharomyces

Mycobacterium

Rhizobium

Escherichia

None

Proinsulin and insulin differ in structure due to the presence of the _____ in proinsulin.

B polypeptide

A polypeptide

C polypeptide

D polypeptide

None

Read the two statements A and B and identify the correct choice from the given options.

Statement A : Agrobacterium tumefaciens is the causative agent of crown gall disease of dicots.

Statement B : Agrobacterium tumefaciens cause infection by entering the plant through wounds and injuries.

Both statements A and B are wrong

Statement A is correct and B is wrong

Both statements A and B are correct

Statement B is correct and A is wrong

None

Which of the following risks are associated with genetically modified foods?

Allergic reactions in human beings

All of these

Antibiotic resistance in microorganisms present in alimentary canal

Toxicity in human beings

None

The Bt toxin is used for killing nematodes in crop plants. But, it is non-toxic to humans. Select the correct reason for this

The Bt-toxin recognises only insect-specific targets.

Conversion of pro Bt-toxin to Bt-toxin takes place in highly alkaline conditions.

The pro Bt-toxin activation requires pH lower than that present in the human stomach.

The pro Bt-toxin activation requires temperature above human body temperature

None

Important objective of biotechnology in agriculture section is

To decrease the seed number

To increase the plant weight

To produce pest resistant varieties of plant

To increase the nitrogen content

None

Assertion : SCID is a primary immunodeficiency.

Reason : It is a serious congenital immunodeficiency.

Both the Assertion and Reason are false

The Assertion is true but the Reason is false.

Both the Assertion and Reason are true but the Reason is not a correct explanation of the Assertion.

Both the Assertion and the Reason are true and the Reason is a correct explanation of the Assertion.

None

10.

Gene can therapy can be referred to as

Cancer treatment using in-vitro cultured stem cells

Pre-clinical testing for inherited diseases in newborns

Treatment of diseases caused by genetic defect

Genetic engineering using rDNA technology

None

11.

What might be an advantage of beginning gene therapy prior to birth?

The body would not reject it as it has not yet recognized 'self'.

This would give the body plenty of time to utilize the new genes.

There probably is not any advantage.

The cells being extremely young. are more receptive of gene therapy.

None

12.

Adenosine deaminase deficiency can be permanently cured by which of the following methods?

Enzymes replacement therapy

Gene therapy at early embryonic stages

All of these

Bone marrow transplantation

None

13.

Transgenic plants are produced by using Ti plasmid from the

E.coli

Agrobacterium tumefaciens

Bacteriophage

Agrobacterium varians

None

14.

Which does not describe a function of the DNA polymerase molecule?

Read the strand of template DNA and recognise the base there

Proofread to ensure that the proper base has been incorporated

Make the proper nucleotide to match with the base read on the template strand

Recognise the free nucleotide that pairs with the base on the template strand of DNA

None

15.

Study the following steps which are followed during the process of gene therapy while treating a patient of SCID

(i) Retrovirus infects lymphocytes extracted from bone marrow of the patient and cultured.

(ii) Engineered cells are injected into patient's bone marrow.

(iii) Normal allele is inserted into a retrovirus.

(iv) Retrovirus makes a DNA copy of its RNA. This DNA carrying the normal allele gets inserted into the chromosome of the host cell.

Arrange the above given steps in correct sequence and select the correct answer.

(iv), (iii), (i), (ii)

(iv), (ii), (iii), (i)

(iii), (i), (iv), (ii)

(iii), (i), (ii) (iv)

None

16.

What is the permanent cure of adenosine deaminase (ADA) deficiency in children?

Infusion of genetically engineered lymphocytes (in which functional ADA-cDNA is introduced) into the patient's blood

Bone marrow transplantation

Introduction of gene isolated from the bone marrow cells which produce ADA, into the cells of the patient at early embryonic stages

Enzyme replacement therapy in which functional ADA is given to patient by injection

None

17.

Some of the common steps involved in the production of humulin are given below. Choose the correct sequence.

I. Synthesis of the gene (DNA) for human insulin artificially.

II. Culturing of recombinant E. coli in bacteria.

III. Purification of humulin.

IV. Insertion of human insulin gene in plasmid DNA.

V. Introduction of recombinant plasmid into E. coli.

VI. Extraction of recombinant gene product from E. coli.

I, V, IV, II ,VI, III

I, IV, V, II ,VI, III

II, I, IV, Ill, V, VI

I, III, V, VI, II, IV

None

18.

Which of the following statements is not correct?

PCR technique is applied to detect HIV in Suspected AIDS patients and to detect mutations in genes in suspected cancer patients

Insulin used for diabetic patients was earlier extracted from pancreas of slaughtered cattle and pigs which was more efficient than that the genetically engineered insulin

Bone marrow transplantation requires periodic infusion of genetically engineered lymphocytes

Bio-remediation is the one of the applications of biotechnology

None

19.

Which of the following protein is produced by the genetically modified microorganism to improve animal milk production?

Bacteriocin

Albumin protein

Bovine somatotropin

None of these

None

20.

What is the function of the polymerase chain reaction in genetic engineering?

Cut DNA into many fragments

Link together newly joined fragments of DNA

Carry DNA into a new cell

Make millions of copies of a specific segment of DNA

None

Time's up